My Microscope configuration.

It would be interesting to know how this microscope was configured at delivery in the somewhat distant past. But since I haven't found the original invoice, let's take a look at what I have in hand today.

Objectives:

Plan 2.5/0.08, Plan 6.3/0.16, Ph1 Plan 16/0.35, Neofluar 25/0.60, Ph2 Neofluar 40/0.75 and a Ph3 Neofluar 100/1.30 oil. This is a decent set of objectives to start a hobby but will likely be replaced over time with the appropriate DIC lenses. Under close examination the glass in some cases shows signs of scratches and the beginning of possible delamination but I can't be sure how this affects contrast and resolution at this time. No complaints, after all this is a used instrument. The plan is to test these objectives and report how well they do in some of the standard diatom tests.

Condenser:

Phako IV 27 apl 0.63. This condenser's N.A. can be reduced to 0.32 by removing the top lens. This condenser is a decent unit but will not get the best out of two highest N.A. objectives. I have access to two other condensers, a 0.90 N.A. and a 1.40 N.A. Again, my plans are to test these condensers with my objective set and report on the results. The condenser is housed in a turret that allows positions of brightfield and Phase contrast I, II and III.





 Camera Adapter:

A two piece camera adapter came with the scope; both offered by Diagnostic Instruments. The base that attaches to the microscope camera port is a Z Clamp while the adapter itself is a 0.60X HRP 60-CMT, designed to work with a ccd camera. My Canon 40D is connected using a T2 adapter. This is not an optimum solution since much of the camera sensor doesn't receive any light from the objective. Ideally, the magnification factor should be in the order of 1.5 to 1.6 to maximize the effectiveness of the camera sensor. The search for another adapter is underway. :)






Oculars (Eyepieces):

These are Kpl W 10X/18  units with extended eye relief so glasses can be worn. Both interpupillary distance and diopter adjustments can be made. I find myself always removing my glasses anyway.






Stage:

This is a rotating stage with geared controls in the X and Y axes.








Lighting:

Lighting is provided by a 12V 100W tungsten bulb attached to the rear of the microscope body. It is powered by a separate 12V power supply.

Accesories:

The microscope is equipped with an Optovar unit. This allows magnifications of 1.25, 1.6 and 2.0. In
addition the unit includes a Bertrand lens which can be used focus and view various planes of the microscope objectives.  This is useful in aligning the phase contrast annuli and plates and also for checking out the condition of objective glass.

There is also a swing-out accessory lens and filter tray under the condenser.